Paclitaxel a549
WebJul 16, 2015 · The human non-small-cell lung carcinoma (NSCLC) cell line, A549, was maintained in Dulbecco’s modified Eagle medium/high glucose (DMEM/H; Thermo Scientific, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; Thermo Scientific, USA), 100 U/mL penicillin (Gibco BRL, Grand Island, NY), and 100 μg/mL streptomycin (Gibco BRL, … WebApr 1, 2000 · A549, H226, and H358 cell lines were cultured for 24 hours in paclitaxel (10 μmol/L) or ZB4 (500 ng/mL), an antagonistic anti-Fas antibody that blocks the activity of Fas receptor, alone. In addition, A549, H226, and H358 cell lines were cultured in the presence of both paclitaxel (10 μmol/L) and ZB4 (500 ng/mL).
Paclitaxel a549
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WebAn shRNA tumor suppressor panel was screened using reverse infection of an A549 tumorigenic cell line and exposing it to a predetermined concentration of paclitaxel, an anticancer drug. WebFeb 19, 2024 · In our previous study, we demonstrated that paclitaxel nanoparticles (PTX-NPs) exhibited greater antitumor activity against A549 cells in vivo and exerted the strongest antitumor effect at 15 hours after light onset (HALO). 23 Moreover, a previous study demonstrated that cell viability at 15 HALO was the lowest among the four time points ...
WebJan 1, 2024 · Parental A549 cell line and taxol-resistant A549/Taxol subline were employed to examine the effect of HEM on taxol resistance. As expected, the IC 50 value of taxol in A549/Taxol was thirty-five times more than that in A549 (Fig. 2A), confirming the taxol resistance of A549/Taxol cells. WebPaclitaxel Inhibits A549 Cell Cycle. Regulating cell cycle distribution is an effective way to treat the proliferation of lung adenocarcinoma cells. The results demonstrated that …
WebThe Bax, caspase-3 protein expression were increased while Bcl-2 protein expression was decreased in A549 cells. Results showed that the polymer containing hydrazone bond is … WebJan 4, 2024 · The results showed that the IC 50 values of paclitaxel in A549, A549/GEM and A549/Paclitaxel cells were 31.441 ± 1.72, 20.22 ± 1.48 and 3193 ± 207.6 nm/ml, respectively, and the IC 50 values of gemcitabine were 481.3 ± 88.35, 8369 ± 474.6 and 406.2 ± 87.11 nm/ml, respectively (Table 1 ).
WebAdditive cytotoxicities, apoptosis and cell cycle changes induced by combination treatment of celecoxib and paclitaxel in A549 NSCLC cell line. Cancer Res. 2004;64:1228. 43. Jiang J, Liu Y, Wu C, et al. Development of drug-loaded chitosan hollow nanoparticles for delivery of paclitaxel to human lung cancer A549 cells.
WebFeb 14, 2024 · The A549 cells both untreated and treated with paclitaxel or CQ or both for different time periods were trypsinized and were seeded in 24 well plates at a density of 200 cells/well. After 24 h when the cells got attached to the substratum, the media was replaced and fresh culture media was added after a PBS wash. bit of land in the sea crosswordWebApr 29, 2024 · Paclitaxel (PTX) is a microtubule-interfering drug that promotes the polymerization of tubulin. 7 Suppression of the microtubules leads to dynamic changes in a cell including mitotic block and cell apoptosis. 8 Targeting drugs to specific cellular pathways that drive cancer cells is a highly promising treatment modality; therefore, a PTX-based … data gatheredWebResults: A549/PTX cells had significant resistance to PTX and higher expression of ABCB1 compared to A549 cells. Apatinib increased the cytotoxicity of PTX, enhanced PTX-induced apoptosis and cycle arrest, and triggered intracellular ROS generation in A549/PTX cells. datagateway powershellWebOct 2, 1997 · Paclitaxel and vinorelbine are synergistic against human breast cancer cells and murine P388 leukemia. We have evaluated the cytotoxicity of both drugs and … data gathering genshin impact discord serverWebA549 cells (2 × 10 4 cells/well) were treated with 100 μM OMT and 10 nM paclitaxel for 24 h. Live cells were stained in green and dead cells were stained in red. The graph (right) shows the... datagather.exe wilcom embroiderystudioWebResults: A549 and A549/T12 cells contain constitutively activated Stat3, and silencing Stat3 by siRNA made both cancer cells more sensitive to paclitaxel. Therefore, PLGA-PEI-TAX-S3SI was synthesized to test its therapeutic role in A549 and A549/T12 cells. Transmission electron microscopy showed the size of PLGA-PEI-TAX-S3SI to be around 250 nm. data gathered through sensesWebPaclitaxel (PTX) is one of the most effective drugs used for the treatment of cancer and is often used in NSCLC chemotherapy. Its remarkable anticancer activities induce cellular ... A549 cells were treated with 13 and 40nM PTX with or without 10lM Andro for 48h. Cells treated with medium plus 0.1% DMSO were used as a control. After treatment ... data gathering analysis example